The deleteriousness effect of acute over-inflation on the endothelium-dependent (ACh) and endothelium-independent (SNP) vasorelaxation. Lean: Zucker lean rats. ZDF: ZDF rats. Rosi: rosiglitazone treatment. 19w: 19 weeks of age. #: ANOVA analysis indicates statistical difference (p < 0.05) of the dose-response curves of ZDF rats between SNP and ACh during acute over-inflation. *: Significant difference (p < 0.05) at the single dose between lean and ZDF rats during acute over-distension.