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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Nitric oxide and superoxide dismutase modulate endothelial progenitor cell function in type 2 diabetes mellitus

Figure 3

mRNA and protein expression of SODs and p22-phox in EPCs. Total RNA and protein of EPCs from type 2 diabetic patients and healthy volunteers were isolated and the mRNA and the protein expressions of a membrane-bound component of NAD(P)H oxidase; p22-phox and the antioxidant enzymes; Cu/ZnSOD, and MnSOD were assessed. (a) Comparison of mRNA expression between p22-phox, Cu/ZnSOD, and MnSOD in EPCs of healthy volunteers (white bars) and type 2 diabetic patients (black bars). (b) Comparison of protein expression between p22-phox, Cu/ZnSOD, and MnSOD in EPCs of healthy volunteers (white bars) and type 2 diabetic patients (black bars). (c) Representative bolts. Blots were scanned and expression of p22-phox, Cu/ZnSOD, and MnSOD was quantified by densitometric analysis and normalized with β-actin. Data are expressed as mean or percentage ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. DM represents diabetic patients.

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