Figure 4

Total GAGs Increased with Culture Time in Both Cells and Medium. Cells were treated with high glucose (empty dot, 30 mM), insulin (solid triangle, 0.01 unit/ml), glucose+insulin (empty triangle) and control (solid dot) for 24, 48, and 72 hours (3 dishes/group). Total GAGs isolated from cells (A), medium (B) and cells +medium (C) and determined by the carbazole assay are shown. A one-tailed t-test was used to determine significant differences between treatment at 24 and 72 hour. Significantly greater than: a, same group at 24 hours; b, glucose at 72 hours; c, insulin at 72 hours; d, glucose+insulin at 72 hours; e, control at 72 hours. p < 0.05.