Figure 2

Treatments preserve HDL antioxidant properties and apolipoprotein A-I-(apo A-I)-PPARγ-mediated gene expression. (a). Fluorescence intensity (arbitrary units) was determined as described in Methods. There was no difference in the fluorescence signal levels measured of the HDL isolated from mice under control diet with respect to that obtained from mice that fed a HFHC diet after 1 day elapsed; thus the average fluorescence value obtained of the HDL from both groups was taken as "Day 1" reference value. Data are mean ± SD from thee separate experiments. (b). ChIP with a PPARγ antibody of study groups were used to amplify an apo A-I promoter fragment. ChIP figures percentages indicate increments or decrements regarding C57BL/6J mice fed HFHC diet. Data of C57BL/6J mice fed chow diet was established as 100%.