A. Expression of the insulin-regulated aminopeptidase (IRAP, 160 KD) and phosphorylation of PKB/Akt (P-Akt, 60 KD) under hyperinsulinemic hyperglycemic conditions, as analyzed by Western blotting. Equal protein amounts were loaded for each sample. B. Phosphorylation of PKB/Akt as estimated by Western blotting. Results were normalized to β-actin. *,**: p < 0.05 for HIHG conditions vs. other insulin concentrations for OxLDL- and mmLDL-treated cells, respectively. Insulin (+: 10 nM, ++: 500 nM), glucose (+: 5.5 mM, ++: 17.5 mM).