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Figure 7 | Cardiovascular Diabetology

Figure 7

From: Saturated free fatty acids and apoptosis in microvascular mesangial cells: palmitate activates pro-apoptotic signaling involving caspase 9 and mitochondrial release of endonuclease G

Figure 7

Palmitate stimulates translocation of the proapoptotic endonuclease G protein by a caspase 9-independent mechanism. (A) HMC were incubated with palmitate (Palm) and palmitate plus the Z-LEHD-FMK caspase 9 inhibitor (C9I, 40 μM) for the times indicated. The cells were fractionated and the level of p33 endonuclease G protein was measured by Western blotting in the cytoplasmic fraction. Equivalent amounts of protein were added to each lane, as confirmed by reprobing with β-actin. (B) Densitometry of cytoplasmic p33 kDa endonuclease G protein from 3 independent experiments. **, P < 0.01, *, P < 0.05 by ANOVA versus 0 time.

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