Palmitate stimulates translocation of the proapoptotic endonuclease G protein by a caspase 9-independent mechanism. (A) HMC were incubated with palmitate (Palm) and palmitate plus the Z-LEHD-FMK caspase 9 inhibitor (C9I, 40 μM) for the times indicated. The cells were fractionated and the level of p33 endonuclease G protein was measured by Western blotting in the cytoplasmic fraction. Equivalent amounts of protein were added to each lane, as confirmed by reprobing with β-actin. (B) Densitometry of cytoplasmic p33 kDa endonuclease G protein from 3 independent experiments. **, P < 0.01, *, P < 0.05 by ANOVA versus 0 time.