Figure 5

Effects of Saxagliptin on adhesion of PACs. A) Representative microphotographs of red-labelled PACs adhering onto HUVEC monolayers (nuclei labelled in bue with Hoechst), using cells from type 2 diabetic patients and healthy controls exposed to Saxagliptin with or without SDF-1α supplementation. B) Quantification of adherent PACs from type 2 diabetic (T2D) and healthy (Ctrl) subjects in the untreated condition and according to the treatment with Saxagliptin, with or without SDF-1α supplementation. *p < 0.05 versus the untreated control condition (Ctrl). C) Effects of Saxagliptin on cell adhesion was determined in cultures of healthy PACs obtained from pre-selected CD14⁺ and CD14^- cells. *p < 0.05 versus the untreated control condition (Ctrl). D) Baseline adhesion and effects of Saxagliptin +/- SDF-1α was tested in separated CD14⁺ and CD14^- PACs, after cells were cultured from unselected PBMCs. *p < 0.05 in CD14⁺ vs CD14^- cells; ^†p < 0.05 versus the control condition.