Figure 2

In vitro DPP-4 inhibition. A) Degrees of soluble DPP-4 inhibition in the conditioned medium by treatment of HUVECs with Diprotin-A (DPA) or different Saxagliptin concentrations, relative to the untreated control condition (CTRL). The experiment was performed in triplicate. *p < 0.05 for ANOVA test. B) Expression of membrane DPP-4 (CD26) on monocytic (MONOs) and lymphocytic (LYMPHs) cells, identified by the side scatted (SSC) versus forward scatter (FSC) morphologic plot of PACs culture. C) Soluble and cellular DPP-4 activity and the extent of its inhibition of Saxagliptin were tested in PACs cultured from CD14^pos and CD14^neg preselected cells. *p < 0.05 for CD14^- versus CD14⁺; †p < 0.05 for cellular versus soluble (n = 3 replicates).