Figure 1

Phenotypic characterization of cultured PACs. A) PACs were characterized for surface marker expression by flow cytometry. In the side scatted (SSC) versus forward scatter (FSC) morphologic plot, lymphocytic cells (LYMPHs) and monocyte-macrophages (MONOs) were identified and gated separately. Histograms reporting the expression of relevant leukocyte (CD45, CD14, CD68) and endothelial markers (CD31, KDR, CD34) are shown, together with mean percent expression from 3 replicates. The red line indicates negative control, while the blue line indicates the stained condition. B) Cells in the PACs culture were stained with the endothelial markers acLDL and Ulex Lectin. The fraction of cells that were positive for both markers were compared in cultures obtained from T2D or healthy control cells. *p < 0.05 T2D vs Ctrl.