Skip to main content
Figure 6 | Cardiovascular Diabetology

Figure 6

From: The compensatory enrichment of sphingosine -1- phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus

Figure 6

The phosphorylation and activation of CERB was induced by N-HDL, G-HDL, rHDL (G-HDL containing S1P). A-C: HUVECs were incubated with 30 μg/ml of N-HDL, G-HDL, rHDL at 15 minute and the activation of CREB was analyzed by Western blotting, HUVECs were pre-incubated with VPC 23019 (15 nmol/ml) for 20 minute and further treated 30 μg/ml of N-HDL, G-HDL, rHDL at 15 minute and the activation of CREB was analyzed by Western blotting (A) and the activation of transcription factor CERB at 15 minute was determined by EMSA (B). Each experiment was repeated three times.

Back to article page

Cardiovascular Diabetology

ISSN: 1475-2840