Changes in pressurised arteriole diameter as responses to increasing concentrations of GKA (RO28-1675). This figure shows the changes in pressurised arteriole diameter (myogenic tone), expressed as a percentage of the maximum diameter (166 ± 6 μm) measured in 0 mM Ca2+/2 mM EGTA buffer. Results are the mean ± SEM from 6 experiments. The upper panel shows the pressurised arteriole diameter responses to increasing concentrations of RO28-1675 or vehicle (DMSO). The * indicates a significant difference (ANOVA, p < 0.05, n = 6) between the RO28-1675 and vehicle treated arterioles. Centre panel, represents the dilatation in response to increasing concentrations of acetylcholine, alone and in the presence of 10 μM RO28-1675. Despite the greater baseline diameter in the presence of 10 μM RO28-1675, the response to acetylcholine was not different. Similarly the lower panel shows the constrictor response to the adrenoceptor agonist phenylephrine, was not different in the presence of 10 μM RO28-1675.