Effect of calcitriol on target genes KLF6, KLF9 and KLF10 mRNA expression in HUVEC stimulated with a diabetic-like environment. HUVEC were incubated for 24 h with HSA (200 μg/ml), AGE-HSA (200 μg/ml) and glucose (250 mg/dl). In addition, 10-10 mol/l calcitriol was given to the cells 1 h after stimulation for an additional 23 h. KLF6, KLF9 and KLF10 mRNA expression were analyzed by real-time PCR and normalized to the GUSB. Data are expressed as mean ± SD of 4–5 independent experiments. *p < 0.05 compared to control group-HSA; **p < 0.05 compared with AGE-Glucose.