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Figure 4 | Cardiovascular Diabetology

Figure 4

From: Vitamin D manipulates miR-181c, miR-20b and miR-15a in human umbilical vein endothelial cells exposed to a diabetic-like environment

Figure 4

Effect of calcitriol on target genes KLF6, KLF9 and KLF10 mRNA expression in HUVEC stimulated with a diabetic-like environment. HUVEC were incubated for 24 h with HSA (200 μg/ml), AGE-HSA (200 μg/ml) and glucose (250 mg/dl). In addition, 10-10 mol/l calcitriol was given to the cells 1 h after stimulation for an additional 23 h. KLF6, KLF9 and KLF10 mRNA expression were analyzed by real-time PCR and normalized to the GUSB. Data are expressed as mean ± SD of 4–5 independent experiments. *p < 0.05 compared to control group-HSA; **p < 0.05 compared with AGE-Glucose.

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Cardiovascular Diabetology

ISSN: 1475-2840