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Figure 5 | Cardiovascular Diabetology

Figure 5

From: Advanced glycation end products potentiate citrated plasma-evoked oxidative and inflammatory reactions in endothelial cells by up-regulating protease-activated receptor-1 expression

Figure 5

Western blot analysis for PAR-1. HUVECs were treated with or without 100 μg/ml AGE-BSA or 100 μg/ml non-glycated BSA for 4 hr. Then proteins were extracted from HUVECs with lysis buffer, separated by SDS-PAGE and transferred to nitrocellulose membranes. Membranes were probed with antibodies raised against PAR-1 or α-tubulin, and then immune complexes were visualized with an enhanced chemiluminescence detection system. Upper panel shows the representative bands of western blots. Lower panel shows the quantitative data. Data were normalized by the intensity of α-tubulin-derived signals and related to the value with non-glycated BSA. N = 3 per group.

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Cardiovascular Diabetology

ISSN: 1475-2840