Figure 7

Detection of 3-nitrotyrosine in mesenteric arteries: 3-nitrotyrosine detection using immunofluorescence was performed in thin sections of NF, or HF arteries using confocal microscopy. Arteries were isolated from LZ rats or from ZDF rats treated or not with ALT-711, Tempol or Tempol + ALT-711. Image contrast and brightness were increased by 10% in each image to improve visibility. Bargraph: Quantification of 3-nitrotyrosine was performed using image density analysis (3–4 arterial sections per artery and 10 rats per group). Mean ± SEM is presented. *P < 0.05, treated versus untreated.