Figure 4

Adiponectin oligomer distribution from different venous collections. Sera collected from the tail vein, portal vein, and left ventricle of 5 mice under anesthesia was gel fractionated by FPLC and oligomer distributions quantified. The tail vein had significantly more LMW and less trimeric adiponectin than the portal circulation. Adiponectin trimers were also reduced at the heart compared to portal vein. Percent abundance was calculated as the summed intensity of peaks by immunoblot over the total intensity of the 24 eluted fractions; analysis by two-tailed student’s t-test with unequal variance. *p < 0.05 as indicated.