Figure 4
From: Kir6.2-containing ATP-sensitive K+ channel is required for cardioprotection of resveratrol in mice
AMPK-Kir6.2/K-ATP channel mediated cardioprotection of resveratrol. (A) Representative immunoblots and quantification of myocardial protein extracts for analysis of total AMPK and p-AMPK obtained from wild type (WT) hearts (n = 6/group). *P < 0.05 vs. WT sham group, #P < 0.05 vs. WT I/R group. (B-D) After preincubation with Compound C (CC, 10 μM) for 30 min followed by resveratrol (Res, 10 μM) 30 min, cardiomyocytes were subjected to OGD device. Cell injuries were assessed by staining with Hoechst 33342 (B-C) and measuring LDH release (D). Scale bar: 100 μm. Arrows point to apoptotic nuclei. Data are represented as means ± SEM from six independent experiments. *P < 0.05 vs. corresponding OGD/R group; #P < 0.05 vs. corresponding OGD/R + Res group. (E-G) WT and Kir6.2-/- (KO) myocytes were treatment with AICAR (50 μM) following subjected to OGD/R device. Cell injuries were assessed by staining with Hoechst 33342 (E-F) and measuring LDH release (G). Data are represented as means ± SEM from six independent experiments *P < 0.01 vs. corresponding control group; #P < 0.01 vs. corresponding OGD/R group.