Figure 7

Expression of analysis of ATOX1, ATP7B and ATP7A in LV tissues from control, diabetic and TETA-treated diabetic rat. A and B: Quantitative analysis of immunofluorescent signal area for protein signals of ATOX1 and ATP7B, respectively. Results were expressed as mean of the percentages of corresponding cross-sectional areas. At least 40 sectional images/group, have been analyzed: **P < 0.01 vs. control. C: RT-qPCR analysis for mRNA levels of Atp7a. Data are means ± SEM and presented relative to the respective controls, which were set at 1. ^##P < 0.01 vs. diabetic: n = 9/group. D: Quantitative analysis of immunofluorescent signal area for ATP7A-protein. Results have been expressed as means of the percentages of corresponding cross-sectional areas. At least 40 sectional images/group, were analyzed: ^##P < 0.01 vs. diabetic. E: Representative confocal images (60 ×-objective) of transverse LV-wall sections labeled with anti-ATP7A antibody (red). Arrows indicate the peri-nuclear stain and Golgi-network structure: n = 40 sectional images/group: scale bar = 30 μm.