TRPV1 activation by dietary capsaicin attenuates endothelial oxidative stress and increases the level of NO in diabetic mice. A and B: Representative protein expressions of p22phox (A) and p-eNOS (B) in aortas from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and wild type mice treated with normal diet (WT Cont). ##P<0.01 versus the WT Cont group; *P<0.05 versus the db/db Cont group. Data are mean ± SEM. Each n = 3. C and D: Representative images and summary data detected by DHE (C) and DAF-2 DA (D) in mesenteric arteries from wild type and db/db mice treated with normal diet (WT Cont and db/db Cont) or normal diet plus 0.01% capsaicin (WT Cap and db/db Cap). #P<0.05 versus WT Cont group; ** P<0.01 versus WT Cap group; ΔΔP<0.01 versus db/db cont group. Data are mean ± SEM. Each n = 4. The scale bar indicates 50 μm.