Figure 1

Representative images of Sirius red staining for total collagen and immunohistochemical staining for collagen I and collagen III in cardiac tissue (200× magnification). Bars represent quantification of expression of total collagen, collagen I and collagen III (% area fraction). TP, L, low-dose triptolide (100 μg/kg/day); TP, M, medium-dose triptolide (200 μg/kg/day); TP, H, high-dose triptolide (400 μg/kg/day). *P < 0.05 versus control; ^#P < 0.05 versus DM, n = 10 per group.