Figure 6

Protein expressions of total eNOS and phosphorylated eNOS at Ser1177 in aortas and hearts. (A) Representative immunoblots of total eNOS, eNOS phosphorylated at Ser1177 and actin in aorta. Control is the common sample used for all Western blots. (B) Quantified total eNOS and phosphorylated eNOS in the aortas and ratio between the phosphorylated and total eNOS in aorta. (C) Representative immunoblots of total eNOS, eNOS phosphorylated at Ser1177 and actin in heart. Control is the common sample used for all Western blots. (D) Quantified total eNOS and phosphorylated eNOS in the hearts and ratio between the phosphorylated and total eNOS. ZL: Zucker lean rats; ZDF: Zucker Diabetic Fatty rats. The number of experiments was 11 and 10 for the ZL and ZDF groups respectively. *: significantly different.