Oxidative stress measurements. (A) Mitochondrial oxidative stress estimated by aconitase-to-fumarase ratio. (B) Cytosolic oxidative stress estimated by lactate-to-pyruvate ratio. (C) Enzymatic activity of glutathione peroxidase (GPx) in the plasma. (D) Antioxidant power of the plasma estimated by the ferric reducing antioxidant power (FRAP) assay. (E) Systemic oxidative stress estimated by the disappearance of the plasma thiol (SH) groups. ZL: Zucker lean rats; ZDF: Zucker Diabetic Fatty rats. The number of experiments was 11 and 10 for the ZL and ZDF groups respectively. *: significantly different.