Expression of tissue kallikrein, kininogen, kinin B2 receptor, ACE2, and Mas. mRNA expression of tissue kallikrein (A), kininogen (B), ACE2 (D), and Mas (E) in cardiac myocytes of control, STZ + Veh, STZ + Alsk, STZ + Benz, STZ + Vals, and STZ + Vals + PD after 10 wks of diabetes was measured by real-time RT-PCR and normalized to 18S mRNA. B2 receptor expression was measured by Western blot analysis (C). A representative Western blot image is shown. Values are expressed as the fold change compared to control ± SEM. *P < 0.05 vs. control, #P < 0.05 vs. STZ + Veh.