Determination of ER stress sensors in wild type, PERK dn, IRE1 dn and ATF6 dn cardiomyocytes. The right side of this figure demonstrates quantitative analysis of immunoblots on the left side of this figure. Columns indicate the expression level of imunoblotted proteins including p-PERK, PERK, p-IRE1, IRE1, cleaved ATF6 and beta- actin respectively. Ratio of p-PERK/PERK, p-IRE1/IRE1 and cleaved ATF6/beta- actin were used to stand for activation levels of PERK, IRE1 and ATF6 in wild type myocytes (black columns) and knocked- down myocytes (grey columns) whether incubated in normal- glucose condition or high- glucose condition. Values in this figure are presented by columns in (mean ± SD) manner. a Values are significantly different from NG. [(&)P < 0.05; (#)P < 0.01; (*)P < 0.001].