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Table 7 Secreted/soluble angiotensin-converting enzyme 2 (ace2) levels in culture media of human cardiomyocytes under normoxia and hypoxia with or without insulin treatment

From: Arg972 Insulin receptor substrate-1 is associated with decreased serum angiotensin-converting enzyme 2 levels in acute myocardial infarction patients: in vivo and in vitro evidence

Group

Normoxia

Hypoxia

 

+Insulin

-Insulin

+Insulin

-Insulin

VC

4.2±1.4

0.8±0.4

19.2±5.3*

1.0±0.5

WT-IRS-1

4.5±1.2

0.9±0.6

20.7±5.5*

1.3±0.7

Arg972-IRS-1

3.8±1.4

0.6±0.4

9.3±3.2*,a,b

0.8±0.3

VC+LY

0.5±0.4

0.5±0.3

0.5±0.3a,b,c

0.5±0.4

SC

4.0±1.1

0.8±0.3

20.3±5.6*

1.1±0.6

IRS-1-shRNA

0.6±0.3

0.5±0.4

0.7±0.5a,b,c

0.6±0.4

  1. Note: Human cardiomyocytes were stably transfected with wild type (WT) IRS-1 or Arg972 IRS-1, or stably transduced with IRS-1-shRNA. Then the cells were cultured under normoxia or hypoxia for 24 hours in the presence or absence of LY294002 (LY, 50 μM) or insulin (1 mg/L). Human cardiomyocytes stably transfected with empty pcDNA3.1 plasmids was used as a control (VC). Secreted/soluble ACE2 levels (ng/mL) in the cell culture media were determined by ELISA. *p<0.05 compared with any group under normoxia; ap<0.05 compared with VC or SC under hypoxia+insulin (1 mg/L); bp<0.05 compared with WT-IRS-1 under hypoxia+insulin (1 mg/L); cp<0.05 compared with Arg972-IRS-1 under hypoxia+insulin (1 mg/L).
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Cardiovascular Diabetology

ISSN: 1475-2840