Interferon regulatory factor-1 together with reactive oxygen species promotes the acceleration of cell cycle progression by up-regulating the cyclin E and CDK2 genes during high glucose-induced proliferation of vascular smooth muscle cells

Table 3 The percentage of phospho-Erk1/2 positive cells

		Uuntransfected VSMCs (%)	VSMCs transfected with pGC-FU (%)	VSMCs transfected with pGCsi-FU-Irf-1 (%)	VSMCs transfected with pGC-FU-Irf-1 (%)
Normal glucose	+	2.56 ± 0.33	2.92 ± 0.28	3.11 ± 0.34	2.65 ± .026
	++	0.32 ± 0.03	0.39 ± 0.04	0.45 ± 0.02	0.24 ± 0.06
Normal glucose + H₂O₂	+	19.7 ± 2.8^	20.5 ± 2.6^	2.89 ± 0.59*	33.7 ± 2.9*^
	++	6.1 ± 0.7^	6.3 ± 0.6^	0.43 ± 0.05*	34.7 ± 2.8*^
High glucose	+	21.8 ± 1.9	23.7 ± 2.2	3.27 ± 0.28*	34.5 ± 3.8*
	++	5.7 ± 0.8	6.2 ± 0.5	0.36 ± 0.03*	35.1 ± 3.2*
High glucose + NAC	+	3.04 ± 0.29^#	3.17 ± 0.42^#	3.13 ± 0.48	2.75 ± .034^#
	++	0.51 ± 0.04^#	0.31 ± 0.06^#	0.48 ± 0.04	0.44 ± 0.05^#

Values are mean ± SD from the determinations repeated in 4 separate experiments (12 samples). For one sample, the percentage of positive cells was determined by counting positive staining cells and total cells in three visual fields (around 3 × 300 cells).
*p < 0.05 vs. corresponding values in untransfected VSMCs; ^P < 0.05 vs. corresponding values in VSMCs under normal glucose; ^#P < 0.05 vs. corresponding values in VSMCs under high glucose.
+ represents positive; ++ represents strong positive.

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