Figure 5
Expression levels of oxidative stress responding transcription factor and uncoupling protein in HUVEC, and HMVEC cells after 24 hours of high glucose exposure. Gene expression of NFE2L2 and UCP1 were measured in (a) HUVEC and (b) HMVEC following 24 hours of exposure to HG-M199. Fold changes were calculated by 2-∆∆CT method using 18s and RPLP0 as housekeeping genes and cells grown in M199 media with 5.6 mM D-glucose as control. *p ≤ 0.06 higher than control for HMVEC.