Figure 5

Effects of propofol on 15 mM glucose-induced Ser⁶⁶⁰phosphorylation of protein kinase Cβ2 (PKCβ2) and PKC activation. (A) Human umbilical vein endothelial cells (HUVECs) were cultured in either 5 mM glucose or 15 mM glucose for 4 h with corresponding treatment. (B) Quantification of protein band density of PKCβ2 or phosphorylated PKCβ2 (*p < 0.05 vs. 5 mM glucose, #p < 0.05 vs. 15 mM glucose, n = 5). ■ represents PKCβ2 phosphorylation, □ represents PKCβ2. (C) HUVECs were cultured in either 5 mM glucose or 15 mM glucose for 4 h with corresponding treatment. (*p < 0.05 vs. 5 mM glucose, #p < 0.05 vs. 15 mM glucose, n = 5). The results were shown as folds increased compared with 5 mM glucose group.