Effects of IFR radiation on eNOS, Akt, p-ERK, p-38 MAPK, VEGF and HO-1 production in cultured EPCs. Administration of IFR radiation on cultured ECFCs for 10, 30, and 40 min followed by treatment of EPCs in high-glucose conditions significantly upregulated high glucose-impaired eNOS production and eNOS activity (p-eNOS/total eNOS). Treatment of IFR therapy for 30, 40 and 60 min also upregulated Akt activation (p-Akt/total Akt). In addition, administration IFR radiation also promoted VEGF production, p-ERK, and p-38 MAPK in high glucose conditions, but not HO-1. Each bar graph shows the summarized data from four separate experiments by densitometry after normalization. Data are means ± SEM; n = 4 in each experiment. (*p < 0.05 compared with control; #p < 0.05 compared with HG group.).