Figure 4
IFR therapy decreased reactive oxidative stress, recovered EPC proliferation, and increased NO production in high-glucose conditions. (A) High glucose markedly increased H2O2 production determined by the relative DCFH-DA fluorescent intensity, and the administration of IFR therapy suppressed high glucose-induced reactive oxidative stress (ROS) index in EPCs culture. (*p < 0.05 compared with control - 0 min; #p < 0.05 compared with high glucose - 0 min) (B) The effect of IFR radiation on EPCs proliferation was analyzed by MTT assay. (*p < 0.05 compared with control - 0 min; #p < 0.05 compared with high glucose - 0 min) (C) Nitrate production (as NO content) in culture medium was measured by Griess reagent. High glucose-suppressed NO production in cultured late EPC s. After 4 days of incubation, IFR radiation increased NO production with or with high glucose conditions. (*p < 0.05 compared with control - 0 min; #p < 0.05 compared with high glucose - 0 min; **p < 0.05 compared with control – 0 min; n = 4 for each experiment).