Effects of far infrared (IFR) therapy on blood flow recovery and new vessels formation in STZ-induced diabetic mice. (A) Representative results of laser Doppler measurements before operation and 4 weeks after hindlimb ischemia surgery in wild-type mice, control (vehicle), IFR therapy, and IFR + NG-nitro-L-arginine methyl ester (L-NAME) mice. Color scale illustrates blood flow variations from minimal (dark blue) to maximal (red) values. Arrows indicate ischemic (right) limb after hindlimb ischemia surgery. Doppler perfusion ratio (ischemic/non-ischemic hind limb) over time in the different groups. Administration of L-NAME in drinking water abolished the beneficial effect of IFR therapy in diabetic mice. (*p < 0.05 compared with DM-control; #p < 0.05 compared with DM-FIR; n = 6) (B) Mice were sacrificed 3 weeks after surgery and capillaries in the ischemic muscles were visualized by anti-CD31 immunostaining. Results are mean ± standard error of mean (SEM). (*p < 0.05 compared with DM-control; #p < 0.05 compared with DM-IFR; n = 6).