Figure 7
From: RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response
Physical interaction between ROCK and moesin proteins is confirmed in HUVECs by immunoprecipitation (IP)- immunoblotting (IB) analysis of HUVEC whole-cell extracts after stimulation with AGE-HSA (50 mg/L,1 h). A: IP with moesin using mouse-antibody and IB detection with goat anti-mouse anti-ROCK antibody, ROCK was detected with or without AGE-HSA stimulation. B: IP with ROCK using rabbit antibody and IB detection with goat anti-mouse anti-moesin antibody, moesin was detected with or without AGE-HSA stimulation. C: IP with phospho-ROCK using rabbit antibody and IB detection with goat anti-mouse anti-moesin antibody, moesin was decreased after AGE-HSA stimulation. BSA was used as control albumin.