Figure 10
From: RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response
Expression moesinT558A decreased the hyper-permeability response (A, n = 3) and F-actin redistribution (B) after AGE-HSA stimulation. HUVECs were stimulated 1 h by 50 mg/L AGE-HSA after transfected with plasmid pcDNA3/HA-moesinT558A for 24 h, or transfected with plasmid pcDNA3/HA-moesinT558D without AGE-HSA stimulation. Representative confocal images of F-actin organization and the formation of stress fiber in HUVECs were showed with similar results observed in 3 different cultures. * P < 0.05 vs control, # P < 0.05 vs AGE-HSA.