Figure 2

Fasudil attenuated high glucose induced VCAM-1 and MCP-1 induction in HUVECS. mRNA expression of VCAM-1 and MCP-1 genes in HUVECs was assessed by real-time PCR. The mRNA level of VCAM-1 and MCP-1 was normalized by GAPDH mRNA level. (A and B) Simultaneous treatment of HUVECs with fasudil for 24 h decreased HG-induced expression of MCP-1(A) and VCAM-1(B). A high concentration of fasudil (10^-5 mM) had a more pronounced effect than the low concentration of fasudil (10^-6 mM). Similar increased concentrations of mannitol did not affect VCAM-1 and MCP-1 expression. * P < 0.05 when compared to control. # P < 0.05 when compared to HG (33 mM) alone. (C and D) Time course of MCP-1(C) and VCAM-1(D) expression: HUVECs were cultured with medium containing 5.5 mM glucose, then the medium was changed to that with HG (20 mM, squares), or HG + fasudil(10^-5 mM, circle) followed by further incubation for the indicated time. Results were expressed as fold induction respect time 0. Means ± SD of three experiments was shown. * P < 0.05 when compared to time 0, # P < 0.05 when compared to HG alone.