Figure 2

Effects of AGEs on late EPC apoptosis. A: Late EPCs were incubated with different concentrations of AGEs (0, 50, 100, 200 and 500 μg/ml) for 24 h. Harvested cells were stained with Annexin V FITC and PI, and quantified by FACS. Right lower quadrant means Annexin V positive and PI negative. Right superior quadrant means Annexin V and PI positive. Left superior quadrant means Annexin V negative and PI positive. Left lower quadrant means Annexin V and PI negative. Annexin V-positive and PI-negative cells were defined as apoptotic cells. B: The proportion of apoptotic cells. Data represent the mean ± SE of four different experiments. *p < 0.05, **p < 0.01 vs. control.