Figure 1

Representative images of EdU staining by different EdU injection methods (magnification ×400). (A) Three intraperitoneal injections of EdU at day 7 after balloon injury (a-l): (a, c, e) EdU staining for Wistar rats at a dose of 5 mg/kg, 25 mg/kg and 50 mg/kg in sequence; (b, d, f) EdU staining for GK rats at a dose of 5 mg/kg, 25 mg/kg and 50 mg/kg in sequence; (g-j) Hoechst 33342 and EdU staining for Wistar and GK rats at a dose of 100 mg/kg in order; (k, l) EdU staining for Wistar and GK rats at a dose of 200 mg/kg, respectively. (m, n) EdU staining for uninjuried Wistar and GK rats at a dose of 100 mg/kg, respectively. (o, p) Hoechst 33342 and EdU staining for GK rats at a single injection of 100 mg/kg, respectively. (B) Line charts depicting the dose dependence of EdU staining. (C) Bar diagrams showing the increased number of EdU-positive cells was more significant in the injury groups than in the uninjured groups for Wistar and GK rats at a dose of 100 mg/kg, respectively. (D) Bar diagrams showing a greater number of EdU-positive cells were observed using multiple injections than a single one. Arrows in images sign EdU-labeled cells and lums indicate luminal side of artery. * indicates Wistar control versus Wistar day 7, # indicates GK control versus GK day 7, $ indicates Wistar day 7 versus GK day 7. p<0.01.