Post translational modifications due to HG. A and B. RyR phosphorylation at S-2808 normalized to total RyR content was significantly enhanced in HG compared to nonHG. C. O-linked N-Acetylglucosamine (O-GlcNAc) was not apparent in myofibrillar proteins except possibly an interfilament protein at ~65 kDa, which was not differentially affected by HG. MW = molecular weight marker. *P<0.05; n=4.