Figure 5

Glibenclamide did not alter expression of IL-1β and TNF-α in cardiac tissue by LPS stimulation in streptozotocin (STZ) diabetic mice. Expression of IL-1β and TNF-α in cardiac tissue were assessed by ELISA 6 h after LPS injection (15 mg/kg, i.p). In non-STZ treated mice, LPS stimulation significantly increased both IL-1β and TNF-α level. In STZ treated mice, LPS stimulation induced greater levels of IL-1β and TNF-α than those in non-STZ treated ones, which were not modified by glibenclamide (5 mg/kg, i.g, × 14 d) pretreatment. *P < 0.05, **P < 0.01 vs control, ^#P < 0.05 vs LPS. Values are means ± SEM (n = 8 per group).