Figure 5

NF-κB activation by TLR2 and TLR4 is augmented in diabetic CAECs. Non-diabetic and diabetic CAECs were stimulated with PGN (10 μg/ml) and LPS (200 ng/ml) for 10, 30, 60 or 120 min. A and B. Representative immunoblots show that stimulation of either TLR2 or TLR4 induced greater phosphorylation of NF-κB p65 in diabetic cells at 30 to 120 min. C. NF-κB p65 was label red by immunostaining with a specific antibody, and nuclei were counter-stained blue with bis-benzimide. Representative immunofluorescent images show more intranuclear NF-κB p65 in diabetic cells at 60 min after stimulation of TLR2 or TLR4.