Figure 6

Stimulation of AGE-pretreated (black squares) HUVEC with heterodimeric S100A8/S100A9 resulted in phosphorylation of both MAP kinases p38 (e) and Erk1/2 (k) within 15 minutes. Representative immunoblots for AGE preincubated HUVEC are shown for phosphorylated p38 (a), total p38 (b), phosphorylated Erk1/2 (f) and total Erk1/2 (g). Control experiments: Phosphorylated p38 (c), total p38 (d), phosphorylated Erk1/2 (h) and total Erk1/2 (i) *p < 0.05 AGE pretreatment vs. albumin-glucose (open circles) for control.