Figure 7

Effect of OCN on ACh-stimulated EDR and PI3K/Akt signaling pathway in descending thoracic aortic strips of ApoE-KO mice fed with HFD. (A) Beneficial effect of OCN treatment for 24 h on EDR. (B) OCN-mediated changes in phosphorylation of eNOS and Akt in the presence of PI3K inhibitor LY294002 or Akt inhibitor V. (C) The effects of OCN, LY294002 and Akt inhibitor V on Akt phosphorylation. (D) The effects of OCN, LY294002 and Akt inhibitor V on eNOS phosphorylation. Values represent mean ± SEM, n = 5-6. ^#P < 0.05, ^##P < 0.01, HF + OCN-treated + LY294002 vs. HF + OCN-treated group; *P < 0.05, **P < 0.01, HF + OCN-treated + Akt inhibitor V vs. HF + OCN-treated group. HF, high fat diet; EDR, endothelium-dependent relaxation; OCN, osteocalcin.