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Figure 2 | Cardiovascular Diabetology

Figure 2

From: Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells

Figure 2

Vaspin inhibits TNFα-induced NF-κB activation in HAECs. The effect of vaspin on TNFα-induced NF-κB promoter activity (A). NF-κB promoter activity was measured at 4 hr after treatment with 100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFα. The effects of vaspin on TNFα-induced activation of p65 (B), and p50 (C). Standardized amounts of p65 (B) and p50 (C) were measured at 1 hr after treatment with 25–100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFα. The effect of vaspin on TNFα-induced IκBα degradation (D and E). Relative levels of phosphorylated IκBα (D) and phosphorylated IκBα (E) were measured at 30 min after treatment with 25–100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFα. Data are mean values ± SEM of five independent experiments. *p < 0.05 vs. untreated cells, †p < 0.05 vs. cells treated with TNFα alone, and §p < 0.05 vs. cells treated with 25 ng/mL vaspin.

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Cardiovascular Diabetology

ISSN: 1475-2840