Figure 3
From: Stiffness memory of EA.hy926 endothelial cells in response to chronic hyperglycemia
Fluorescent staining of F-actin. Cells cultured in HG medium: P5 (A), P10 (B), P14 (C), P15 (D), P20 (E), P24 (F) and for the cells moved to NG after 14 passages in hyperglycemic environment (P14+)P2 NG (G), (P14+)P6 NG (H), (P14+)P10 NG (I). The images are organized in such a way, that images (D), (E), (F) can be easily compared to (G), (H), (I), respectively. Initially, cell present normal structure of actin cytoskeleton (arrow 1). Later, the polymerization of F-actin leads to stress fibers formation (arrow 2). Additionally, numerous intercellular gaps are formed (arrow 3). Arrow 4 indicate visible actin depolymerization. Images are representative of n=20 independent experiments.