Figure 1

Methylglyoxal-stimulated${\mathrm{O}}_2^{•-}$production in EA. hy926 endothelial cells. A.${\mathrm{O}}_2^{•-}$ generation determined in EA.hy296 endothelial cells treated with different concentrations of MG (0, 50, 100 and 200 μM) for 0, 2, 4, 6 and 8 h respectively. Data are arithmetic means ± SEM (n = 6). * indicates significant difference (p < 0.05) from the absence of MG (ANOVA). B.${\mathrm{O}}_2^{•-}$ generation determined in EA.hy296 endothelial cells treated with MG (100 μM) for 8 h in the absence (Control) or in the presence of L-NAME (50 μM), BH4 (100 μM), NH4 (100 μM) or sepiapterin (20 μM) in the last 4 h of MG treatment or 24-h pre-treatment with 300 μM Tempol and 50 μM L-NAME (ONOO^- suppression) prior to the addition of MG. Data are arithmetic means ± SEM (n = 6). * indicates significant difference (p < 0.05) from Control (ANOVA).