Table 2 Real-time PCR primers
Primer | Forward | Reverse | Amplicon | Annealing |
|---|---|---|---|---|
|  |  |  | size (kb) | temp (°C) |
ANP | TTCCGGTACCGAAGATAACAGCCA | TGACACACCACAAGGGCTTAGGAT | 91 | 60 |
BNP | AGACAAGGGAGAACACGGCATCAT | ACAGAATCATCTGGGACAGCACCT | 85 | 60 |
HDAC1 | TTCCTGCGTTCTATTCGCCCAGAT | AACAAGCCATCAAACACCGGACAG | 98 | 60 |
HDAC2 | TACAACAGATCGCGTGATGACCGT | TCCCTTTCCAGCACCAATATCCCT | 94 | 62 |
α-skeletal actin | TTGTGCACCGCAAATGCTTCTAGG | GCAACCACAGCACGATTGTCGATT | 90 | 60 |
α-cardiac actin | TGTAGGTGATGAAGCCCAGAGCAA | TGGTGCCAGATCTTCTCCATGTCA | 105 | 60 |
β-myosin heavy chain | TGGCTGGTGAGGTCATTGACAGAA | TGGCTGGTGAGGTCATTGACAGAA | 104 | 60 |
GAPDH | TGTGATGGGTGTGAACCACGAGAA | CATGAGCCCTTCCACAATGCCAAA | 133 | Per plate |