Figure 7

Effects of GATA-4 specific siRNA on phenylephrine (PE)-induced cell contraction in H9c2 cells. The high glucose-treated H9c2 cells were incubated with siRNA-GATA-4 (Si) or scrambled RNA as a control (Scramble) for 24 hr before PE challenge and then their planar surface area was measured. The upper panel (A) shows a representative change of cells in each group. The lower panel (B) expresses the differences between the four groups as the mean ± SEM (n = 6 per group). The high glucose-treated group differed markedly from the control incubated in normal medium (***P < 0.001). The high glucose-treated GATA-4 RNAi group differed markedly from the scrambled RNA-treated group treated with a high glucose concentration (^##P < 0.01, ^###P < 0.001).