Fig. 5

Decreased size of adrenal medulla and altered expression in diabetic Hif1aCKO mice. A Representative confocal images of cross-sections through the right adrenal glands of control and Hif1aCKO, diabetic and non-diabetic adults were labeled with anti-tyrosine hydroxylase (TH). Hoechst-stained cell nuclei. Scale bar = 500 µm. B TH⁺ area was quantified using ImageJ and expressed as a percentage of the total adrenal gland area. Data are mean ± SEM (n = 5 gland per genotype and all sections per adrenal gland were used). C-F Representative Western blots and quantification of CHgA and HIF-2α protein from the adrenal glands. Beta actin was used as a loading control. Data are mean ± SD (n = 5–6 individual adrenal glands). G Relative mRNA expression levels of phenylethanolamine N-methyltransferase (Pnmt) and Th by qRT-PCR. mRNA was extracted from whole adrenal glands (n = 6–8). Data are mean ± SEM. Statistical significance assessed by two-way ANOVA followed by post hoc Tukey ‘s multiple comparisons test, and unpaired t-tests (G); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. AU, arbitrary units