Fig. 10

Adverse tissue remodeling in the myocardium of the left ventricle of diabetic Hif1aCKO. A Representative confocal images of immunohistochemical staining of macrophages (F4/80) and C WT1 with Hoechst-stained cell nuclei in the LV of the heart. White arrows indicate F4/80⁺ cells and WT1⁺ cells. Scale bars 50 µm. B Quantification of number of F4/80⁺ cells and D WT1⁺ cells in the LV. Data are mean ± SEM (n = 4 samples per genotype, one section per sample and 6 areas per section). Statistical significance assessed by two-way ANOVA followed by post hoc Tukey ‘s multiple comparisons test ****P < 0.0001. LV, left ventricle