Fig. 6

TGF-β and IFN-γ mediates CCL18/CD4 T cell-induced lipolysis

FC of in vitro adipocyte lipolysis induced by CM obtained from T cells stimulated with and without CCL18, in the presence and absence of IFN-γ (a) or TGF-β (b) blocking antibodies (n = 10). FC of the lipolysis in adipocytes stimulated with and without rIFN-γ (c) in the presence or absence of blocking IFN-γ antibodies (n = 9); and treatment with rTGF-β combined with TGF-β blocking antibodies (n = 6) is shown in (d). Statistical significance was evaluated by one-way ANOVA with multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. UMAPs for IFNG and TGFB expression in CD4⁺ T cell clusters are shown in (e). Dots are colored according to the expression levels, going from darker purple for low expression to yellow for high expression. Circles represent different clusters. Circle color corresponds to the color of the populations represented in the boxes.