Fig. 5

Transcriptome analysis reveals differential cardiac gene expression due to loss of Tbc1d4. Whole heart transcriptome analysis via RNA sequencing and subsequent Ingenuity pathway analysis A Volcano Plot of differentially regulated genes due to the loss of Tbc1d4 displaying the size of differential regulation and size of significance. B Differentially regulated canonical pathways and potential upstream regulators of Ingenuity Pathway Analysis/IPA for differentially regulated genes with adjusted p-value < 0.01. C Differentially regulated genes contained in over-represented Reactome pathways and IPA disease annotations regarding altered metabolic processes. Overlap of genes and corresponding p-value of CPDB over-representation analysis are indicated above the respective heatmap Data presented as Z-Score. Significance threshold was set at p < 0.01 (n = 4). WT wild type, D4KO Tbc1d4-knockout