Fig. 1
Deletion of Tbc1d4 leads to reduced cardiac GLUT4 content and impaired ex vivo glucose uptake. A mRNA copy number, normalized by Tbp expression, of Tbc1d1 and Tbc1d4 in whole heart tissue and isolated left ventricle. B Heart weight/body weight ratio of WT and D4KO animals on standard diet. C Total cardiac glycogen content (n = 7–8); D, E Cardiac protein abundance of TBC1D4, GLUT1 and GLUT4 (n = 7), F Insulin-stimulated [3H]-2-deoxyglucose uptake in isolated cardiac left-ventricular papillary muscle (n = 5–6). Data presented as mean ± SEM. Statistical analysis was performed using unpaired two-tailed student’s t-Test (A-C,E; WT vs. D4KO: *p < 0.05; **p < 0.01; ***p < 0.001) or two-way ANOVA with Bonferroni’s multiple comparison test (F; basal vs. insulin: *p < 0.05; WT vs. D4KO: #p < 0.05); male mice, 36 weeks of age. WT wild type, D4KO Tbc1d4-knockout